Evaluation of IL-12 production inducing activity (in vitro)

When the plant-origin nano-sized lactic acid bacterium SNK and OK432* were compared, the activities were found to be at the same level.
*OK432: Immunotherapeutic agent to increase the immunity of the human body.

(Measurement method)
•Method of measurement for IL-12 production inducing activity
•Using BALB/c mouse: 23 weeks of age
•The concentration of samples at the time of measurement: 10 μg/mL after converting to cell weight

Inhibition of Th17 cell production (Peyer’s patch of mouse ileum)

With the continuous use of the plant-origin nano-sized lactic acid bacterium SNK, the behavior of the immune system cells developing intestinal problems such as colitis was studied.

As a result, a significant dose-dependent inhibition of SNK in the production of Th17 cells, which is relates autoimmune diseases, was observed.

(Test Method)

30 mice were divided into 3 groups of 10 mice (C: only physiological saline solution, L: physiological saline solution + SNK 2mg/kg, H: physiological saline solution + SNK 10mg/kg) and were administered for 15 days. After oral administration for 10 days, dextran sodium sulfate, which induces colitis, was injected to cause inflammation. After dissecting 16 days after administration, analyses for each group were conducted to determine the ratios of Th17 cells and the gene expressions of transcription factor that are related to Th17 cells.